The Ultimate Guide to Creating a Dual-Extracted Lion’s Mane Tincture

Lions Mane Tinctures

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Lion’s Mane mushrooms (Hericium erinaceus) have gained widespread recognition for their potential cognitive benefits, such as improved memory, focus, and overall brain health. To fully harness the power of Lion’s Mane, creating a dual-extracted tincture is an effective method, as it ensures that both the alcohol-soluble and water-soluble compounds are extracted and made bioavailable. In this comprehensive guide, we will walk you through the step-by-step process of creating your own potent Lion’s Mane dual-extracted tincture.

What is a Dual-Extracted Tincture?

A dual-extracted tincture is a liquid extract that combines both alcohol and water extraction methods. This ensures that a wide range of beneficial compounds are extracted from the mushroom. Lion’s Mane mushrooms contain both water-soluble compounds, such as beta-glucans, and alcohol-soluble compounds, such as hericenones and erinacines, which contribute to its cognitive-enhancing properties. By using a dual-extraction method, you can maximize the potential benefits of Lion’s Mane.

Materials and Ingredients for recipe

To make a Lion’s Mane dual-extracted tincture, you will need the following materials and ingredients:

  1. Fresh or dried Lion’s Mane mushrooms
  2. A high-proof alcohol (such as vodka, brandy, or ethanol)
  3. Distilled water
  4. A clean glass jar with a tight-fitting lid
  5. A fine mesh strainer or cheesecloth
  6. A funnel
  7. Amber glass dropper bottles for storage

Step 1: Prepare the Lion’s Mane Mushrooms

Begin by preparing your Lion’s Mane mushrooms. If you are using fresh mushrooms, clean them gently with a soft brush or cloth to remove any debris. Next, chop the mushrooms into small pieces to increase the surface area for extraction.

Lions mane for dual-extracted lion's mane tinctures.
Fresh Lions mane cut up into pieces.

If you are using dried Lion’s Mane mushrooms, you can either grind them into a powder using a coffee grinder or break them into smaller pieces by hand.

Step 2: Alcohol Extraction

Place the prepared Lion’s Mane mushrooms into a clean glass jar. Pour enough high-proof alcohol over the mushrooms to cover them completely, filling the jar about halfway. Seal the jar with a tight-fitting lid, and label it with the date and contents.

Lions mane alcohol extraction.
Lions mane with a high proof alcohol.
Lions mane alcohol extraction in jar for dual-extracted lion's mane tinctures.
Lions mane alcohol extraction process has started.

Store the jar in a cool, dark place for at least 4 weeks, or up to 8 weeks for a stronger extraction. Shake the jar every few days to ensure the alcohol is evenly distributed and effectively extracts the alcohol-soluble compounds.

Step 3: Strain the Alcohol Extract

After 4-8 weeks, use a fine mesh strainer or cheesecloth to strain the alcohol extract into a clean glass jar or container, separating the liquid from the mushroom material. Squeeze the mushroom material to extract as much liquid as possible, and then set aside the strained alcohol extract.

Alcohol extraction for lion's mane dual-extracted tinctures.
Strained lion’s mane alcohol extraction.

Step 4: Water Extraction

Take the strained mushroom material and place it into a stove pot. Add about 5 times the amount of alcohol extraction of water into the pot. Bring the mushrooms and water to a boil and then simmer for about 4-5 hours. This simmering process will help extract the water-soluble compounds from the mushrooms.

Step 5: Strain the Water Extract

After simmering, let the mixture cool down, then strain the water extract into a clean glass jar or container, just as you did with the alcohol extract. Discard the spent mushroom material.

Step 6: Combine the Extracts

Combine the alcohol and water extracts in a glass jar or container, using a funnel if necessary. The final mixture should have an alcohol content of at least 25% to ensure proper preservation. You can use a hydrometer to measure the alcohol content, and adjust the ratio of alcohol to water extracts as needed. For example, if your alcohol extract is very strong, you may need to add more water extract to achieve the desired alcohol content.

Water extraction and alcohol extraction for lions mane dule-extracted tinctures
Alcohol extraction and water extraction. (Dual extraction)

Step 7: Reduce and Concentrate (Optional)

If you prefer a more concentrated tincture, you can gently heat the combined extracts in a double boiler or a heat-resistant container placed in a pot of simmering water. Be careful not to let the temperature rise too high, as this could cause the alcohol to evaporate. Reduce the liquid to your desired concentration, then allow it to cool.

Step 8: Store and Bottle the Tincture

Transfer the cooled and combined extracts to amber glass dropper bottles using a funnel. Amber bottles protect the tincture from light, which can degrade its potency over time. Label the bottles with the contents and the date of preparation.

Lions Mane Dual-Extracted Tinctures
Two cups of Dual extracted liquid generates 7 2-oz dropper bottles.

Store the bottles in a cool, dark place, such as a cabinet or pantry. The dual-extracted Lion’s Mane tincture should have a shelf life of at least 1-2 years when stored properly.

How to Use Your Lion’s Mane Dual-Extracted Tincture

To experience the cognitive benefits of Lion’s Mane, start by taking a small dose of the tincture (about 1/4 to 1/2 dropper) and placing it under your tongue or adding it to a beverage, such as tea or water. Gradually increase the dosage over time as desired, up to a full dropper (or as recommended by a healthcare professional). Individual responses to Lion’s Mane can vary, so it’s essential to listen to your body and adjust the dosage accordingly.


Creating your own Lion’s Mane dual-extracted tincture is a rewarding process that allows you to harness the full range of benefits that this remarkable mushroom has to offer. By following the steps outlined in this guide, you can experience the cognitive-enhancing properties of Lion’s Mane and support your overall brain health. As research into this fascinating fungus continues, we may discover even more reasons to appreciate its complex and captivating properties.

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